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 ND-1000 Applications - Proteins and Labels
| The NanoDrop ® ND-1000 Spectrophotometer can be used to simultaneously measure the concentrations of proteins (A280) and up to two fluorescent dyes. In addition to measuring both components of a protein-dye conjugate, the versatile Proteins and Labels module can also be used to measure the purity of metalloproteins (such as hemoglobin) using wavelength ratios. |
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Key Features
- Six different reference types available : 1 abs=1mg/ml, BSA, IgG, Lysozyme, user entered mass extinction coefficient, user entered molar extinction coefficient
- Measurement of wide variety of fluorescent dyes possible: Cy3, Cy5, Cy3.5, Cy5.5, Alexas 488, 546, 555, 594, 647 and 660
- Concentration of protein and up to two dyes calculated and displayed
- Reblank feature allows for the re-analysis of previous sample with a new blank
- Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
- Sample ID field is key-focused for easy bar coder use
- Absorbance is represented as a 1mm path equivalent
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Measurement concentration range
Sample Type |
Detection Limit
(pmol/ul) |
Approximate Upper Limit (pmol/ul) |
Typical Reproducibility
(minimum 5 replicates)
(SD= pmol/ul; CV= %) |
Cy3, Cy3.5, Alexa Fluor 555, Alexa Fluor 660
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0.20 |
100 |
sample range 0.20-4.0 pmol/ul: ± 0.20 pmol/ul sample range >4.0 pmol/ul: ± 2% |
Cy5, Cy5.5 and Alexa Fluor 647
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0.12 |
60 |
sample range 0.12-2.4 pmol/ul: ± 0.12 pmol/ul sample range >2.4 pmol/ul: ± 2% |
Alexa Fluor 488 and Alexa Fluor 594
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0.40 |
215 |
sample range 0.40-8.0 pmol/ul: ± 0.40 pmol/ul sample range >8.0 pmol/ul: ± 2% |
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0.30 |
145 |
sample range 0.30-6.0 pmol/ul: ± 0.30 pmol/ul sample range >6.0 pmol/ul: ± 2% |
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