  |
|
 ND-1000 Applications - Protein A280
| The NanoDrop® ND-1000 Spectrophotometer will accurately and reproducibly measure purified protein samples up to 100 mg/ml (BSA), without dilution. To do this, the instrument detects the high concentration and automatically utilizes the 0.2mm pathlength to calculate the absorbance. A sample measurement output is shown below. |
 |
Key Features
- Six different reference types available : 1 abs=1mg/ml, BSA, IgG, Lysozyme, user entered mass extinction coefficient, user entered molar extinction coefficient
- Concentration of protein is calculated and displayed as mg/ml
- Reblank feature allows for the re-analysis of previous sample with a new blank
- Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
- 260/280 ratio calculated and displayed
- Sample ID field is key-focused for easy bar coder use
- Absorbance is represented as a 10mm path equivalent
|
Measurement concentration range
Sample Type |
Detection Limit |
Approximate Upper Limit |
Typical Reproducibility
(minimum 5 replicates)
(SD= mg/ml; CV= %) |
|
0.10 mg/ml |
100 mg/ml |
sample range 0.05-10 mg/ml: ± 0.10 mg/ml
sample range >10mg/ml: ± 2% |
|
|
