Microporation is a novel electroporation technology developed by Digital Bio Technology and supplied by Labtech International. Microporation is unique in replacing the cuvette electrode with a pipette tip-style chamber as the electroporation space. This unique technology minimises heat generation and oxide formation and ensures a uniform electric field (factors responsible for cell death in conventional elecroporation). Great transfection efficiency and cell viability are only the beginning of the benefits from the new Microporation Technology.....
We have found the microporator ideal for transfection of dissociated neurons.
Transfection efficiencies are at least as good as those achieved with lipofection,
biolistic transformation or other electoporators. In addition, the very small volumes,
and hence cell numbers, required for the microporator means that from a practical
and ethical point of view this is the machine of choice for neuronal transfection in our lab. |


