Synergy™ HT Multi-Detection Microplate Reader
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Specifications| Detection method | Fluorescence, Time Resolved Fluorescence, Luminescence: Photomultiplier tube Absorbance: Silicon photodiode |
| Read method | Fluorescence and Luminescence: Endpoint, kinetic, well area scanning Absorbance: Endpoint, kinetic, well area scanning, spectral scanning |
| Microplate types | 6- to 384-well plates PCR tube trays Terasaki trays |
| Reading speed | Fluorescence and Luminescence: 35 sec/96 wells (depends on settings) Absorbance: 15 sec/96 wells (depends on settings) |
| Wavelength range | Fluorescence: Excitation 300-650 nm (200-700 nm with TR model), Emission 300-700 nm (red extended option available) Time Resolved Fluorescence: Excitation 200-700 nm, Emission 300-700 nm (red extended option available) Luminescence: 300-700 nm (red extended option available) Absorbance: 200 to 999 nm, tunable in 1 nm increments |
| Wavelength selection | Fluorescence: Deep blocking interference filters Time Resolved Fluorescence: Monochromator Absorbance: Monochromator |
| Monochromator wavelength accuracy | +/- 2 nm |
| Monochromator wavelength repeatability | +/- 0.2 nm |
| Filter wheel capacity | Fluorescence: 4 excitation positions, 4 emission positions Absorbance: N/A |
| Filters supplied | Fluorescence: 4 excitation filters, 4 emission filters Absorbance: N/A |
| Bandpass | Fluorescence: Variable, dependent on filter Time Resolved Fluorescence: 10 nm Absorbance: 2.4 nm |
| Dynamic range | Fluorescence: 0 - 99,999 RFU's Time Resolved Fluorescence: 0 - 99,999 RFU's Luminescence: 0-99,999 RLU's Absorbance: 0 - 4.0 OD |
| OD accuracy | < 1% at 2.0 OD typical < 3% at 3.0 OD typical |
| OD linearity | < 1% from 0 to 3.0 OD typical |
| OD repeatability | < 0.5% at 2.0 OD typical |
| Resolution | 0.001 OD |
| Sensitivity | Sodium fluorescein 2.5 pg/ml (1.3 fmol/well) typical* Sodium fluorescein 1 pg/ml (0.5 fmol/well) limit of detection* Luciferase 0.1 pg/well typical ATP 100 amol/well limit of detection (*Black plate, top measurement, 3mm diameter probe) |
| Standard probes | 3 mm top, 5 mm bottom. Top probe height and top or bottom reading position are software adjustable/selectable (fluorescence). Other probe sizes available. |
| Light source | Fluorescence: Tungsten Halogen 1,000 hrs lifetime Time Resolved Fluorescence: Xenon flash lamp, 1 billion flashes lifetime Absorbance: Xenon flash lamp, 1 billion flashes lifetime |
| Stray light | Absorbance: 0.03% at 230 nm typical |
| Temperature control | 4oC above ambient to 50oC |
| Shaking | Linear shaking: Low, med, high and variable speeds |
| Reagent dispensers | 2 syringe pumps |
| Dispense volume | 5-1000 µl in 1 µl increments |
| PC Software | Gen5 software. Control through USB or serial port. |
| Power | 100-240 VAC 50/60 Hz |
| Dimensions | 16"W x 15"D x 10"H (40.6 x 38 x 25.4 cm) |
| Weight | 40 lbs (18 kg) |
| Regulatory | For in vitro diagnostic use (Except in the European Union). All BioTek microplate instrumentation is CE and ETL marked. For details on approvals and standards compliance, please contact BioTek. |
| Automation | Compatible with Bio-Stack Microplate Stacker and Bio-Stack Twister II Microplate Handler. |
Specifications subject to change.
